Nanoflow LC is widely used for high resolution separations and high sensitivity MS detection. The Agilent 1260 Infinity Nanoflow LC system incorporates Agilent's unique Electronic Flow Control (EFC) with active feedback and real time flow adjustment for constant flow delivery to the column independent of system backpressure. After its first introduction with Agilent capillary and nanoflow pumps, EFC with real time flow control is the gold standard for reliable and robust nanoflow performance. Easy to use, the Agilent 1260 Infinity Nanoflow LC simply requires the user to input the desired nanoflow set-point to obtain the precise flow and gradient performance required for retention time reproducibility and stable ion generation essential for high sensitivity nanoflow LC/MS performance.